Paul McGinley, captain of the 2014 European team, is promoting the campaign after seeing how volunteers influenced previous tournaments.
"It takes a huge team of people to make the Ryder Cup a successful event, and the thousands of volunteers involved are a very important part of that. We are always appreciative of anyone who gives their time to volunteer and support golf and there are not many countries in the world better than Scotland for producing volunteers and marshals whose golf knowledge is second to none.
"We will be looking for volunteers who have a knowledge of the game but also people who will enjoy the experience and help to make the Ryder Cup an unforgettable event for the fans who will have a vital role to play in trying to help the European team retain the trophy at Gleneagles."
The application process is now open online and closes on April 13. It includes a search for 60 volunteers to help out at next year's Junior Ryder Cup at Blairgowrie Golf Club, also in Perthshire.
Successful applicants, however, have to pay £75 for the food, drink and clothing they will be given at the event, organisers say.